Amplitaq gold manual

AmpliTaq Gold or 3 U AmpliTaq, respectively. In the manual hot-start procedure, AmpliTaq was added to the reaction mix - ture at 94°C during initial denaturation. Finally, the master mixture was divided into three µL aliquots, and 1- µL cDNA of KM-H2 cells, PBMC or stimulated PBMC was added. All time-release PCRs were carried out in a µL re-Cited by: and AmpliTaq Gold DNA Polymerases in conventional PCR and AmpliTaq in manual hot-start www.doorway.ru genomic DNA extracted from bloodstains and semen stains was amplified with 1 U of DNA polymerase in a µL reaction as follows: (A) AmpliTaq, according to the manufacturer’s recom -. AmpliTaq Gold is derived from a recombinant, thermostable, 94 kDa DNA poly-merase encoded by a modified form of the Thermus aquaticus DNA polymerase gene which has been inserted into an Escherichia coli host.7 This ultra-pure, stable polymerase is available in a variety of package sizes with GeneAmp 10X PCR GoldFile Size: KB.

AmpliTaq Gold® Master Mix was designed for ° coverage of Availability: In stock. AmpliTaq Gold PCR Master Mix contains everything required for successful PCR amplification in one convenient package, with all components premixed and premeasured. AmpliTaq Gold Master Mix was designed for coverage of a full ranExonuclease Activity: 5'-3'. AmpliTaq Gold ® Master Mix. Product Insert. AmpliTaq Gold ® Master Mix Quick. Reference Card. Applied Biosystems Veriti ™ Thermal. Cycler User Guide. GeneAmp ® PCR System Base. Module User’s Manual. GeneAmp® PCR System Well Sample Block Module. Part number. AmpliTaq. For detailed procedures, refer to the AmpliTaq Gold® Master Mix Protocol (PN ). List of components All solutions are prepared in deionized, filter-sterilized water.

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